Association of toll-like receptor 4 gene (Thr399Ile) polymorphism with type II diabetes mellitus patients
1
Department of clinical Laboratory Sciences, Faculty of Pharmacy, University of Kufa, Iraq, Iraq
2
Department of Pharmaceutics and industrial pharmacy, Faculty of Pharmacy, University of Kufa, Iraq, Iraq
Submission date: 2025-03-24
Final revision date: 2025-06-11
Acceptance date: 2025-06-20
Publication date: 2025-07-30
Corresponding author
Ahmed J. Mohammed
Department of clinical Laboratory Sciences, Faculty of Pharmacy, University of Kufa, Iraq, Iraq
Department of clinical Laboratory Sciences, Faculty of Pharmacy, University of Kufa, Iraq, Iraq
Wiadomości Lekarskie 2025;(7):1381-1388
KEYWORDS
Genetic susceptibilityIraqi populationType 2 diabetes mellitus (T2DM)TLR4 gene polymorphismThr399Ile (rs4986791)
TOPICS
ABSTRACT
Aim:
Background: Diabetes mellitus (DM) is a chronic condition marked by high blood glucose levels due to impaired insulin production or action, posing a significant global health challenge. Toll-like receptor 4 (TLR4) polymorphisms have been implicated in inflammation and type 2 diabetes mellitus (T2DM). Aims: This study investigates the association of the TLR4 Thr399Ile (rs4986791) polymorphism with T2DM in Iraqi patients and its influence on clinical parameters.
Material and methods:
Materials and Methods: A case-control study was conducted on 200 participants (100 T2DM cases and 100 healthy controls). Phenotypic analyses included fasting blood glucose, HbA1c, lipid profiles, and insulin sensitivity indices. Genotyping of the TLR4 Thr399Ile polymorphism was performed using PCR-RFLP. Statistical analyses were conducted using SPSS.
Results:
Results: The CT and TT genotypes were significantly more frequent in T2DM patients, associated with higher odds ratios (OR=2.40, p=0.003; OR=5.33, p=0.04, respectively). HDL levels were significantly lower in variant genotypes (p = 0.01). Other clinical parameters showed no significant genotype-specific differences.
Conclusions:
Conclusion: The TLR4 Thr399Ile polymorphism is significantly associated with increased T2DM risk and altered HDL levels in Iraqi patients, highlighting its potential as a genetic marker for early risk assessment.
Background: Diabetes mellitus (DM) is a chronic condition marked by high blood glucose levels due to impaired insulin production or action, posing a significant global health challenge. Toll-like receptor 4 (TLR4) polymorphisms have been implicated in inflammation and type 2 diabetes mellitus (T2DM). Aims: This study investigates the association of the TLR4 Thr399Ile (rs4986791) polymorphism with T2DM in Iraqi patients and its influence on clinical parameters.
Material and methods:
Materials and Methods: A case-control study was conducted on 200 participants (100 T2DM cases and 100 healthy controls). Phenotypic analyses included fasting blood glucose, HbA1c, lipid profiles, and insulin sensitivity indices. Genotyping of the TLR4 Thr399Ile polymorphism was performed using PCR-RFLP. Statistical analyses were conducted using SPSS.
Results:
Results: The CT and TT genotypes were significantly more frequent in T2DM patients, associated with higher odds ratios (OR=2.40, p=0.003; OR=5.33, p=0.04, respectively). HDL levels were significantly lower in variant genotypes (p = 0.01). Other clinical parameters showed no significant genotype-specific differences.
Conclusions:
Conclusion: The TLR4 Thr399Ile polymorphism is significantly associated with increased T2DM risk and altered HDL levels in Iraqi patients, highlighting its potential as a genetic marker for early risk assessment.
| eISSN: | 2719-342X |
| ISSN: | 0043-5147 |
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