Aim: This research was conducted to assess the prevalence of ocular dryness during the course of systemic isotretinoin in acne patients in Iraq. Materials and Methods: 64 acne patients using systemic isotretinoin in doses ranging from 20mg to 60 mg were enrolled in this study. All patients had been checked for ocular dryness by using Schirmer’s test before starting therapy and after each month regularly for 6 times. Results: 64 acne patients were included in this study with a high percentage of the female gender (81.75%) with a mean age of 21.2± 3.4. The mean duration of systemic isotretinoin is 4.3 months while the mean total cumulative dose is 5.8 grams. About 31.25% among the participants have developed ocular dryness, and the most frequent grade is grade III (50%).70% of patients that have ocular dryness use lubricant eye drops. However, no significant correlations were seen between dosage, gender, duration, age, and TCD with ocular dryness. Conclusions: This research reveals that ocular dryness may occur during the course of systemic isotretinoin. This research contributes to the raise the awareness of the effects of isotretinoin use on the health of the ocular surface. However, no significant correlations were seen between dosage, gender, duration, age, and TCD with ocular dryness.