Aim: To investigate the correlations between the level of IL1β mRNA gene expression and the type and duration of infertility and to study the levels of IL1β mRNA gene expression in women with endometriosis associated with infertility. Materials and Methods: For mRNA gene expression analysis IL1β and determination of relative normalized mRNA expression IL1β used the real-time reverse transcription polymerase chain reaction method (RT-PCR).. Examined group consists of 30 infertile women undergoing assisted reproductive technologies. The main group consisted of 20 women diagnosed with endometriosis undergoing assisted reproductive technologies. The control group consisted of 10 healthy women. Results: In the main group, the level of IL1β mRNA gene expression before preparing was 26.7877±0.01, which was significantly higher than the level after preparing (0.1610±0.01* ). Analized results, it’s found out that Mean level of mRNA IL1β in women with endometriosis associated infertility 1-st degree is 8.53 c.u., at the same time Mean level of mRNA IL1β in women with endometriosis associated infertility 2-nd degree is 1.0 c.u. Conclusions: The inclusion of probiotics in a comprehensive regimen of preparation for assisted reproductive technologies leads to a noticeable improvement in the patient's well-being.