Effect of Ocimum basilicum Herbs Extract on Pro-Inflammatory Cytokines in Ethanol-Induced Liver Damage in Rats
1
Department of Biology, College of Science for Women,, University of Baghdad, Baghdad, Iraq., Iraq
2
University of Kirkuk, Kirkuk, Iraq,, College of Veterinary Medicine, University of Kirkuk, Kirkuk, Iraq,, Iraq
3
Department of Pathology and Poultry disease, College of Veterinary Medicine, University of Kirkuk, Kirkuk, Iraq, Iraq
4
Basic science department, Al- Iraqia university, Iraq
5
Department of Chemistry and Biochemistry, Faculty of Medicine, Al Muthanna University, Iraq
Submission date: 2025-04-12
Final revision date: 2025-06-20
Acceptance date: 2025-07-20
Publication date: 2025-08-30
Corresponding author
Karima Akool AlSalihi
Basic science department, Al- Iraqia university, Baghdad, 16375, Baghdad, Iraq
Basic science department, Al- Iraqia university, Baghdad, 16375, Baghdad, Iraq
Wiadomości Lekarskie 2025;(8):1522-1529
KEYWORDS
TOPICS
ABSTRACT
Aim:
To evaluates the hepatoprotective effect of Ocimum basilicum extract in minimizing ethanol-induced liver damage in rats by its action on pro-inflammatory cytokine levels.
Material and methods:
A total of 120 male rats were divided into four groups: a control group, an ethanol-induced liver damage group, a low-dose basil treatment group, and a high-dose basil treatment group. Pro-inflammatory cytokines (TNF-α, IL-6, IL-1β) were measured using ELISA.
Results:
The ethanol exposure group showed significantly elevated TNF-α (548.8 ± 83.78 pg/mL), IL-6 (410.3 ± 167.7 pg/mL), and IL-1β (373.1 ± 127.7 pg/mL) compared to the control group. Basil supplementation, particularly at high doses (100 mg/kg), effectively reduced these cytokines, with TNF-α (316.4 ± 57.37 pg/mL) and IL-6 (133.9 ± 86.76 pg/mL) levels approaching control values. IL-1β was also significantly reduced (152.3 ± 79.37 pg/mL) but remained slightly elevated.
Conclusions:
This study showed that Ocimum basilicum extract has potent hepato protective and anti-inflammatory properties, making it a promising natural therapeutic agent for alcohol-induced liver damage.
To evaluates the hepatoprotective effect of Ocimum basilicum extract in minimizing ethanol-induced liver damage in rats by its action on pro-inflammatory cytokine levels.
Material and methods:
A total of 120 male rats were divided into four groups: a control group, an ethanol-induced liver damage group, a low-dose basil treatment group, and a high-dose basil treatment group. Pro-inflammatory cytokines (TNF-α, IL-6, IL-1β) were measured using ELISA.
Results:
The ethanol exposure group showed significantly elevated TNF-α (548.8 ± 83.78 pg/mL), IL-6 (410.3 ± 167.7 pg/mL), and IL-1β (373.1 ± 127.7 pg/mL) compared to the control group. Basil supplementation, particularly at high doses (100 mg/kg), effectively reduced these cytokines, with TNF-α (316.4 ± 57.37 pg/mL) and IL-6 (133.9 ± 86.76 pg/mL) levels approaching control values. IL-1β was also significantly reduced (152.3 ± 79.37 pg/mL) but remained slightly elevated.
Conclusions:
This study showed that Ocimum basilicum extract has potent hepato protective and anti-inflammatory properties, making it a promising natural therapeutic agent for alcohol-induced liver damage.
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| ISSN: | 0043-5147 |
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