Aim: To assess hormonal status and vitamin D metabolic features in women of reproductive age presenting with abnormal uterine bleeding and associated extragenital conditions. Materials and Methods: The study was conducted at the clinical base of the Department of Obstetrics, Gynecology, Pediatric Gynecology, and Medical Genetics of Kharkiv National Medical University (KhNMU). A total of 110 women of reproductive age were examined and divided into two clinical groups. The first group (main group, MG) included 80 patients diagnosed with abnormal uterine bleeding (AUB) in combination with extragenital cconditions. The second group (control group, CG) consisted of 30 somatically healthy women who presented for routine preventive examinations. Hormonal status was assessed by measuring serum concentrations of luteinizing hormone (LH), follicle-stimulating hormone (FSH), progesterone (PRG), estradiol (E2), and vitamin D using enzyme-linked immunosorbent assay (ELISA) methods. Statistical analysis was performed using the general-purpose software package Statistica 6.0. Results: The average age of the participants was 31.5 ± 13.5 years. Among women in the main group, the most frequently observed extragenital conditions were diseases of the endocrine, circulatory, and digestive systems. Analysis of serum hormone levels revealed a decrease in FSH (1.84-fold), LH (1.64-fold), and PRG (2.6-fold) (KWT, p < 0.01). Conversely, E2 levels were elevated by 3.52-fold in the MG (KWT, p < 0.01). Serum vitamin D levels in the MG were reduced by 2.25- fold compared to the CG (KWT, p < 0.01). A statistically significant (p < 0.05) positive correlation was found between serum E2 and vitamin D levels (r = 0.61). Conclusions: The study revealed distinct features of the hormonal profile and vitamin D metabolism in women of reproductive age with abnormal uterine bleeding and concomitant extragenital conditions. Specifically, this patient population demonstrated decreased serum levels of LH, FSH, PRG, and vitamin D, accompanied by hyperestrogenemia. A statistically significant correlation was observed between serum E2 and vitamin D levels.