Aim: To conduct a comparative analysis of the motor activity regimen and daily energy expenditure of cadets and employees in police investigative units. Materials and Methods: The research involved cadets of the 1st-4th training years (n = 86) and employees of police investigative units (n = 64). To establish the levels of motor activity and daily energy expenditure of cadets and employees in police investigative units, the Framingham method was used based on the registration of activities during the day. Results: A significant difference in cadets’ motor activity index and daily energy expenditure during their educational activities and police investigators during their service activities (p < 0.001) was found. It was found that during the day, the cadets consumed 2658.8 ± 39.6 kcal in the process of their educational activities with their motor activity index at 34.43 ± 0.32 points. The employees showed 2412.4 ± 35.1 kcal of daily energy expenditure with their motor activity index at 31.18 ± 0.29 points. Conclusions: The results obtained are due to the specifics of the educational activities of cadets and the peculiarities of the service activities of police inves tigators, which are determined by an extended stay in a forced position and a low level of physical activity. The results of the conducted research should be taken into account when organizing the physical training of police investigators in the course of their service activities.