Aim:
Aims: to evaluate nephroprotective effects of Coenzyme Q10 in renal ischemia -reperfusion injury in rats by targeting Notch-1 signaling pathway.

Material and methods:
Materials and Methods: 28 male Wistar albino rats were randomly allocated into four groups (7 rats/group): sham group (laparotomy, without ischemia), control group (30 min. ischemia/ 2 hours reperfusion), olive oil group (rats injected intraperitoneally. with 0.1 ml olive oil once daily for 2 days, 1 hour after the administration of olive oil in 3rd day renal ischemia was induced), Coenzyme Q10 group (rats injected with Coenzyme Q10 (10 mg/kg) intraperitoneally. once daily for 2 days, 1 hour after the administration of Coenzyme Q10 in 3rd day renal ischemia was induced). Kidneys were harvested after 2 hours of reperfusion for assessment of kidney injury molecule-1, interleukin 1 β, TNF α, F2 Isoprostane, glutathione, and caspase 3 by ELISA technique, Notch-1, Jagged-1 mRNA level were assessed by reverse transcription-polymerase chain reaction.

Results:
Results: Coenzyme Q10 significantly ameliorated renal ischemia -reperfusion injury as evidenced by the significant reduction of of kidney injury molecule-1, interleukin 1 β, TNF α, F2 Isoprostane, and caspase 3 in rat kidneys pretreated with Coenzyme Q10, glutathione level was significantly elevated in rat kidneys pretreated with Coenzyme Q10. Notch-1 and Jagged-1 mRNA expression were significantly decreased compared to control group.

Conclusions:
Conclusion: Coenzyme Q10 provide nephroprotection against renal ischemia reperfusion injury owing to its antioxidant, anti-inflammatory, and anti-apoptotic properties, those effects may be linked to the downregulation of Notch-1 and Jagged-1.