Aim:
Aims: This study was performed to investigate the potential nephroprotective effect of Tangeretin on bilateral renal I/R injury in male rats.

Material and methods:
Materials and methods: Forty male rats were split into four groups of ten (sham, control, DMSO, and Tangeretin). The sham group underwent a median laparotomy under anaesthesia without inducing ischemia/reperfusion; the control group underwent clamping for thirty minutes on the bilateral renal artery, followed by two hours of reperfusion; the vehicle group received DMSO one hour before induction of ischemia; and the Tangeretin group received 5 mg/kg of Tangeretin one hour before ischemia. Biochemical parameters (KIM1, IL-1β, and TNF-α, F2-isoprostane, GSH, and caspase-3) were measured using an ELISA approach. Furthermore, histological alterations were examined, and the Notch/Jagged1 signalling pathway was assessed using quantitative reverse transcriptase polymerase chain reaction (qRT-PCR).

Results:
Results: Tangeretin pre-treatment reduced kidney damage molecules (KIM1, IL-1β, and TNF-α, F2-isoprostane, GSH, and caspase-3) while increasing antioxidant indicators and decreasing inflammatory and apoptotic markers. Improving histological outcomes and significantly decreasing Notch1 and Jagged-1 gene expression in kidney tissues during renal ischemia/reperfusion injury.

Conclusions:
Conclusion: Tangeretin has significant nephroprotective advantages in renal IRI by decreasing the Notch pathway and exhibiting anti-apoptotic, antioxidant, and anti-inflammatory properties.