Assessment of Local Immunity Markers in Patients with Chronic Rhinosinusitis and Biofilms in the Upper Airway Mucosa
1
Department of Rhinology, Allergology with the group of Radiology, Kolomiychenko Otolaryngology Institute of National Academy of Medical Sciences of Ukraine, Ukraine
2
Department of Pathophysiology and Immunology, Kolomiychenko Otolaryngology Institute of National Academy of Medical Sciences of Ukraine, Ukraine
Submission date: 2025-08-18
Final revision date: 2025-12-11
Acceptance date: 2026-01-13
Publication date: 2026-04-30
Corresponding author
Yurii Maliarenko
Department of Rhinology, Allergology with the group of Radiology, Kolomiychenko Otolaryngology Institute of National Academy of Medical Sciences of Ukraine, Zoolohichna, 3, 03057, Kyiv, Ukraine
Department of Rhinology, Allergology with the group of Radiology, Kolomiychenko Otolaryngology Institute of National Academy of Medical Sciences of Ukraine, Zoolohichna, 3, 03057, Kyiv, Ukraine
Wiadomości Lekarskie 2026;(4):788-793
KEYWORDS
TOPICS
ABSTRACT
Aim:
To comprehensively evaluate local immune markers in patients with chronic rhinosinusitis (CRS) with and without biofilms in the upper airway mucosa, and to determine pathogenetically significant immune alterations associated with biofilm presence that may contribute to chronic inflammation and reduced treatment effectiveness.
Material and methods:
Oropharyngeal secretion was analyzed in 20 CRS patients (with and without biofilms) and 8 healthy controls. Levels of interleukin-1β, α-interferon, secretory IgA, immune complexes, and cellular composition were evaluated. Biofilms were detected via SYTO9/propidium iodide fluorescent staining.
Results:
CRS patients with biofilms showed significantly reduced α-interferon levels and increased concentrations of immune complexes. IL-1β and sIgA levels did not differ between CRS subgroups. All CRS patients exhibited reduced epithelial cell counts and increased neutrophil percentages.
Conclusions:
The study demonstrates that low α-interferon levels and high immune complex concentrations in patients with CRS and biofilms represent pathogenetically significant immune alterations. These findings highlight the need for further investigation of local and systemic immune mechanisms that support biofilm persistence and may open perspectives for more effective therapeutic strategies.
To comprehensively evaluate local immune markers in patients with chronic rhinosinusitis (CRS) with and without biofilms in the upper airway mucosa, and to determine pathogenetically significant immune alterations associated with biofilm presence that may contribute to chronic inflammation and reduced treatment effectiveness.
Material and methods:
Oropharyngeal secretion was analyzed in 20 CRS patients (with and without biofilms) and 8 healthy controls. Levels of interleukin-1β, α-interferon, secretory IgA, immune complexes, and cellular composition were evaluated. Biofilms were detected via SYTO9/propidium iodide fluorescent staining.
Results:
CRS patients with biofilms showed significantly reduced α-interferon levels and increased concentrations of immune complexes. IL-1β and sIgA levels did not differ between CRS subgroups. All CRS patients exhibited reduced epithelial cell counts and increased neutrophil percentages.
Conclusions:
The study demonstrates that low α-interferon levels and high immune complex concentrations in patients with CRS and biofilms represent pathogenetically significant immune alterations. These findings highlight the need for further investigation of local and systemic immune mechanisms that support biofilm persistence and may open perspectives for more effective therapeutic strategies.
| eISSN: | 2719-342X |
| ISSN: | 0043-5147 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
